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cd3 t cell fraction  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd3 t cell fraction
    Cd3 T Cell Fraction, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd3+t+cell+fraction/CD3%CE%B5+Antibody%2C+anti-mouse%2C+REAdye_lease/pmc10692662-337-4-30
    Average 99 stars, based on 5 article reviews
    cd3 t cell fraction - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Staining:

    Article Title: TRAIL-dependent apoptosis of peritoneal mesothelial cells by NK cells promotes ovarian cancer invasion
    Article Snippet: .. To further divide the CD3 + T cell fraction into CD3+/CD4+ T helper cells and CD3+/CD8+ cytotoxic T-cells, the preselected CD14 − lymphocytes were stained with APC-labelled anti-human CD8 antibody (Miltenyi Biotec), incubated with anti-APC microbeads and isolated via MACS. ..

    Incubation:

    Article Title: TRAIL-dependent apoptosis of peritoneal mesothelial cells by NK cells promotes ovarian cancer invasion
    Article Snippet: .. To further divide the CD3 + T cell fraction into CD3+/CD4+ T helper cells and CD3+/CD8+ cytotoxic T-cells, the preselected CD14 − lymphocytes were stained with APC-labelled anti-human CD8 antibody (Miltenyi Biotec), incubated with anti-APC microbeads and isolated via MACS. ..

    Isolation:

    Article Title: TRAIL-dependent apoptosis of peritoneal mesothelial cells by NK cells promotes ovarian cancer invasion
    Article Snippet: .. To further divide the CD3 + T cell fraction into CD3+/CD4+ T helper cells and CD3+/CD8+ cytotoxic T-cells, the preselected CD14 − lymphocytes were stained with APC-labelled anti-human CD8 antibody (Miltenyi Biotec), incubated with anti-APC microbeads and isolated via MACS. ..

    Article Title: CTB linked autoantigens induce dendritic cell tolerance for protection against diabetes autoimmunity
    Article Snippet: White blood cells (WBCs) were separated from red blood cells (RBC) and platelet fractions with RBC lysis buffer as described above and the WBCs were pelleted at (2,000 RPM), for 10 min at 4oC, in a Beckman Coulter Allegra X-15R centrifuge, equipped with an SX4750 rotor. .. The CD3+ T cell fraction was positively selected from the WBCs using the MACS Pan T Cell Isolation Kit II protocol for indirect magnetic isolation of naïve T cells from human blood, as described by the manufacturer (Miltenyi Biotec. ..

    Article Title: Human immune response towards recombinant Helicobacter pylori urease and cellular fractions.
    Article Snippet: Vaccination against Helicobacter pylori is of particular clinical interest.. Recombinant urease, the major protein in H. pylori, has been used for mucosal vaccination trials in different animal models, but was found to be ineffective in humans.. The current study therefore investigated the human immune response towards recombinant H. pylori urease A and B (rUreA/B) expressed in E. coli compared to different cellular fractions of H. pylori (cytosol, total, inner and outer membrane).

    Article Title: Allergen-driven suppression of thiol production by human dendritic cells and the effect of thiols on T cell function.
    Article Snippet: Dendritic cells are a major source of extracellular thiols needed for T cell activation, a process in which CD40mediated stimulation plays a pivotal role.. The Dermatophagoides pteronyssinus group 1 mite allergen (Der p 1) has previously been shown to cleave CD40 from the surface of human dendritic cells, thereby suggesting that Der p 1 might compromise the ability of these cells to sustain thiol production during T cell activation.. This has therefore prompted us to examine the effect of the mite protease allergen Der p 1 on thiol production by human dendritic cells.

    Magnetic Cell Separation:

    Article Title: TRAIL-dependent apoptosis of peritoneal mesothelial cells by NK cells promotes ovarian cancer invasion
    Article Snippet: .. To further divide the CD3 + T cell fraction into CD3+/CD4+ T helper cells and CD3+/CD8+ cytotoxic T-cells, the preselected CD14 − lymphocytes were stained with APC-labelled anti-human CD8 antibody (Miltenyi Biotec), incubated with anti-APC microbeads and isolated via MACS. ..

    Article Title: Lineage-specific chimerism monitoring after allogeneic haematopoietic stem cell transplantation: do we really know what we are measuring?
    Article Snippet: .. Data were collected on 151 blood samples received from 84 adult patients who had undergone allogeneic HSCT at Sheffield Teaching Hospitals NHS Foundation Trust between 14 April 2014 and 21 October 2014 and who had subsequently undergone chimerism assessment of a CD3 T-cell fraction using the Magnetic-Activated Cell Sorting (MACS) system (Miltenyi Biotech Ltd, Bisley, UK). .. Following separation, 0 1 ml of the fraction was analysed by flow cytometry to determine the CD3 T-lymphocyte purity via direct immunofluorescence (FACSCanto II benchtop flow cytometer; Becton Dickinson, Oxford, UK) with antibodies directed against CD2 (Becton Dickinson) to circumvent the possibility of the CD3 binding sites being masked due to free anti-CD3 in the MACS separation.

    Article Title: CTB linked autoantigens induce dendritic cell tolerance for protection against diabetes autoimmunity
    Article Snippet: White blood cells (WBCs) were separated from red blood cells (RBC) and platelet fractions with RBC lysis buffer as described above and the WBCs were pelleted at (2,000 RPM), for 10 min at 4oC, in a Beckman Coulter Allegra X-15R centrifuge, equipped with an SX4750 rotor. .. The CD3+ T cell fraction was positively selected from the WBCs using the MACS Pan T Cell Isolation Kit II protocol for indirect magnetic isolation of naïve T cells from human blood, as described by the manufacturer (Miltenyi Biotec. ..

    Article Title: Lineage-specific chimerism monitoring after allogeneic haematopoietic stem cell transplantation: do we really know what we are measuring?
    Article Snippet: .. Data were collected on 151 blood samples received from 84 adult patients who had undergone allogeneic HSCT at Sheffield Teaching Hospitals NHS Foundation Trust between 14 April 2014 and 21 October 2014 and who had subsequently undergone chimerism assessment of a CD3+ T-cell fraction using the Magnetic-Activated Cell Sorting (MACS) system (Miltenyi Biotech Ltd, Bisley, UK). .. Following separation, 0 1 ml of the fraction was analysed by flow cytometry to determine the CD3+ T-lymphocyte purity via direct immunofluorescence (FACSCanto II benchtop flow cytometer; Becton Dickinson, Oxford, UK) with antibodies directed against CD2 (Becton Dickinson) to circumvent the possibility of the CD3 binding sites being masked due to free anti-CD3 in the MACS separation.

    FACS:

    Article Title: Lineage-specific chimerism monitoring after allogeneic haematopoietic stem cell transplantation: do we really know what we are measuring?
    Article Snippet: .. Data were collected on 151 blood samples received from 84 adult patients who had undergone allogeneic HSCT at Sheffield Teaching Hospitals NHS Foundation Trust between 14 April 2014 and 21 October 2014 and who had subsequently undergone chimerism assessment of a CD3 T-cell fraction using the Magnetic-Activated Cell Sorting (MACS) system (Miltenyi Biotech Ltd, Bisley, UK). .. Following separation, 0 1 ml of the fraction was analysed by flow cytometry to determine the CD3 T-lymphocyte purity via direct immunofluorescence (FACSCanto II benchtop flow cytometer; Becton Dickinson, Oxford, UK) with antibodies directed against CD2 (Becton Dickinson) to circumvent the possibility of the CD3 binding sites being masked due to free anti-CD3 in the MACS separation.

    Article Title: Lineage-specific chimerism monitoring after allogeneic haematopoietic stem cell transplantation: do we really know what we are measuring?
    Article Snippet: .. Data were collected on 151 blood samples received from 84 adult patients who had undergone allogeneic HSCT at Sheffield Teaching Hospitals NHS Foundation Trust between 14 April 2014 and 21 October 2014 and who had subsequently undergone chimerism assessment of a CD3+ T-cell fraction using the Magnetic-Activated Cell Sorting (MACS) system (Miltenyi Biotech Ltd, Bisley, UK). .. Following separation, 0 1 ml of the fraction was analysed by flow cytometry to determine the CD3+ T-lymphocyte purity via direct immunofluorescence (FACSCanto II benchtop flow cytometer; Becton Dickinson, Oxford, UK) with antibodies directed against CD2 (Becton Dickinson) to circumvent the possibility of the CD3 binding sites being masked due to free anti-CD3 in the MACS separation.

    Cell Isolation:

    Article Title: CTB linked autoantigens induce dendritic cell tolerance for protection against diabetes autoimmunity
    Article Snippet: White blood cells (WBCs) were separated from red blood cells (RBC) and platelet fractions with RBC lysis buffer as described above and the WBCs were pelleted at (2,000 RPM), for 10 min at 4oC, in a Beckman Coulter Allegra X-15R centrifuge, equipped with an SX4750 rotor. .. The CD3+ T cell fraction was positively selected from the WBCs using the MACS Pan T Cell Isolation Kit II protocol for indirect magnetic isolation of naïve T cells from human blood, as described by the manufacturer (Miltenyi Biotec. ..

    Article Title: The vitamin D analog, TX527, promotes a human CD4+CD25highCD127low regulatory T cell profile and induces a migratory signature specific for homing to sites of inflammation.
    Article Snippet: .. In some experiments, CD4+ T cells were further separated from the total CD3+ T cell fraction after the 10-d culture period by negative selection (CD4+ T cell Isolation Kit II, Miltenyi Biotec, purity .95%). ..

    Article Title: Allergen-driven suppression of thiol production by human dendritic cells and the effect of thiols on T cell function.
    Article Snippet: Dendritic cells are a major source of extracellular thiols needed for T cell activation, a process in which CD40mediated stimulation plays a pivotal role.. The Dermatophagoides pteronyssinus group 1 mite allergen (Der p 1) has previously been shown to cleave CD40 from the surface of human dendritic cells, thereby suggesting that Der p 1 might compromise the ability of these cells to sustain thiol production during T cell activation.. This has therefore prompted us to examine the effect of the mite protease allergen Der p 1 on thiol production by human dendritic cells.

    Selection:

    Article Title: Human immune response towards recombinant Helicobacter pylori urease and cellular fractions.
    Article Snippet: Vaccination against Helicobacter pylori is of particular clinical interest.. Recombinant urease, the major protein in H. pylori, has been used for mucosal vaccination trials in different animal models, but was found to be ineffective in humans.. The current study therefore investigated the human immune response towards recombinant H. pylori urease A and B (rUreA/B) expressed in E. coli compared to different cellular fractions of H. pylori (cytosol, total, inner and outer membrane).

    Article Title: The vitamin D analog, TX527, promotes a human CD4+CD25highCD127low regulatory T cell profile and induces a migratory signature specific for homing to sites of inflammation.
    Article Snippet: .. In some experiments, CD4+ T cells were further separated from the total CD3+ T cell fraction after the 10-d culture period by negative selection (CD4+ T cell Isolation Kit II, Miltenyi Biotec, purity .95%). ..

    Article Title: Allergen-driven suppression of thiol production by human dendritic cells and the effect of thiols on T cell function.
    Article Snippet: Dendritic cells are a major source of extracellular thiols needed for T cell activation, a process in which CD40mediated stimulation plays a pivotal role.. The Dermatophagoides pteronyssinus group 1 mite allergen (Der p 1) has previously been shown to cleave CD40 from the surface of human dendritic cells, thereby suggesting that Der p 1 might compromise the ability of these cells to sustain thiol production during T cell activation.. This has therefore prompted us to examine the effect of the mite protease allergen Der p 1 on thiol production by human dendritic cells.



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    Image Search Results


    ( A ) Purified CD3 + CD4 + T cells were labeled with CFSE and cultured as described in ± TMV or DC-derived MV (5 µg/mL). On days 3, 5 and 8, the frequency of CD4 + CD25 + FOXP3 + Treg among proliferating T cells was determined by flow cytometry. The data (means ± SD) represent three independent experiments (*p<0.01). ( B ) Proliferating CD3 + CD4 + T cells (squares) were tested for co-expression of CD25 in a representative co-culture ± TMV. A higher proportion of proliferating CD4 + T cells expressed CD25 in the co-culture with TMV than without TMV. ( C ) The proliferating CD4 + CD25 + T cells in the co-cultures with TMV were evaluated for the frequency of FOXP3 + T cells upon gating on the CD4 + CD25 high subset (see box). Over 90% of these cells also expressed intracellular FOXP3. Data are representative for one out of 6 cultures tested.

    Journal: PLoS ONE

    Article Title: Tumor-Derived Microvesicles Induce, Expand and Up-Regulate Biological Activities of Human Regulatory T Cells (Treg)

    doi: 10.1371/journal.pone.0011469

    Figure Lengend Snippet: ( A ) Purified CD3 + CD4 + T cells were labeled with CFSE and cultured as described in ± TMV or DC-derived MV (5 µg/mL). On days 3, 5 and 8, the frequency of CD4 + CD25 + FOXP3 + Treg among proliferating T cells was determined by flow cytometry. The data (means ± SD) represent three independent experiments (*p<0.01). ( B ) Proliferating CD3 + CD4 + T cells (squares) were tested for co-expression of CD25 in a representative co-culture ± TMV. A higher proportion of proliferating CD4 + T cells expressed CD25 in the co-culture with TMV than without TMV. ( C ) The proliferating CD4 + CD25 + T cells in the co-cultures with TMV were evaluated for the frequency of FOXP3 + T cells upon gating on the CD4 + CD25 high subset (see box). Over 90% of these cells also expressed intracellular FOXP3. Data are representative for one out of 6 cultures tested.

    Article Snippet: Total CD3 + CD4 + T cell fractions or isolated CD4 + CD25 + cells were cultured in AIMV medium with plate-bound OKT3 (1 µg/mL; American Type Culture Collection), soluble anti-CD28 Abs (1 µg/mL) and IL-2 (150 IU/mL) at 37°C/5%CO 2 in wells of 96-wells plates.

    Techniques: Purification, Labeling, Cell Culture, Derivative Assay, Flow Cytometry, Expressing, Co-Culture Assay

    ( A ) Flow cytometry analysis of TGF-β1 and IL-10 expression on TMV purified from OVCAR-3 SN and coated onto latex beads. ( B ) CD4 + CD25 high FOXP3 + T cells were cultured with OKT3, anti-CD28 and IL-2 (150 IU/mL) +/− TMV for 72 h at 37°C in the presence of Golgistop and then stained for CD4, CD3, CD25 and intracellular TGF-β1 and IL-10. Expression of both cytokines was up-regulated in the presence of TMV (p<0.05). ( C ) SMAD2/3 and STAT3 phosphorylation in Treg before and after exposure to TMV. Representative results are from one of three independent experiments for A , B and C . ( D ) The percentage of CD4 + CD25 high FOXP3 + T cells increased among CD4 + CD25 + T cells cultured in the presence of TMV but not DC-derived MV. Neutralizing anti-TGF-β1 and/or anti-IL-10 Abs inhibited the induction of Treg by TMV. Non-blocking IgG isotype control Abs were used as controls. Asterisks indicate decreases (p<0.05) in Treg percentages in the presence of neutralizing Abs. Results are means ± SD of three independent experiments.

    Journal: PLoS ONE

    Article Title: Tumor-Derived Microvesicles Induce, Expand and Up-Regulate Biological Activities of Human Regulatory T Cells (Treg)

    doi: 10.1371/journal.pone.0011469

    Figure Lengend Snippet: ( A ) Flow cytometry analysis of TGF-β1 and IL-10 expression on TMV purified from OVCAR-3 SN and coated onto latex beads. ( B ) CD4 + CD25 high FOXP3 + T cells were cultured with OKT3, anti-CD28 and IL-2 (150 IU/mL) +/− TMV for 72 h at 37°C in the presence of Golgistop and then stained for CD4, CD3, CD25 and intracellular TGF-β1 and IL-10. Expression of both cytokines was up-regulated in the presence of TMV (p<0.05). ( C ) SMAD2/3 and STAT3 phosphorylation in Treg before and after exposure to TMV. Representative results are from one of three independent experiments for A , B and C . ( D ) The percentage of CD4 + CD25 high FOXP3 + T cells increased among CD4 + CD25 + T cells cultured in the presence of TMV but not DC-derived MV. Neutralizing anti-TGF-β1 and/or anti-IL-10 Abs inhibited the induction of Treg by TMV. Non-blocking IgG isotype control Abs were used as controls. Asterisks indicate decreases (p<0.05) in Treg percentages in the presence of neutralizing Abs. Results are means ± SD of three independent experiments.

    Article Snippet: Total CD3 + CD4 + T cell fractions or isolated CD4 + CD25 + cells were cultured in AIMV medium with plate-bound OKT3 (1 µg/mL; American Type Culture Collection), soluble anti-CD28 Abs (1 µg/mL) and IL-2 (150 IU/mL) at 37°C/5%CO 2 in wells of 96-wells plates.

    Techniques: Flow Cytometry, Expressing, Purification, Cell Culture, Staining, Derivative Assay, Blocking Assay